A comparative study on the different staining methods. Use millipore filtered water in the waterbath and staining procedure. Mycobacterium and many nocardia species are called acidfast because during an acidfast staining procedure. The lipoid capsule of the acid fast organism takes up carbolfuchsin and resists decolorization with a dilute acid rinse.
Acidfast stain principle, procedure, interpretation and examples. For confirmation of bacterial presence and identity, we used standard staining including gram and acidfast staining 33, 34. Acid fastness is an uncommon characteristic shared by the genera mycobacterium and nocardia weakly acid fast. Pdf staining techniques for detection of acid fast bacilli. By editorial team on january 11, 2020 in bacteriology, microbiology. The nature of the acidfast stain pubmed central pmc.
The ziehlneelsen stain zn stain, also called the hot method of afb staining, is a type of differential bacteriological stain used to identify acid fast organisms, mainly mycobacteria. Acid fast staining was developed by robert koch in 1882 and later modified by other scientists. Acid fast stain principle, procedure, interpretation and examples. Ziehlneelsen zn method of acid fast staining technique is used to stain mycobacterium species including m. Get a printable copy pdf file of the complete article 809k, or click on a page image below to browse page by page. Protocols for using the acidfast stain in the microbiology lab. Number of specimens for the detection of acid fast bacilli. He developed a stain for the bacterium, although it was not very effective for visualizing this slender bacillus. The acid fast stain robert koch was the first person to isolate and identify mycobacterium tuberculosis from a patient with tuberculosis. Full text full text is available as a scanned copy of the original print version. Used in the demonstration of acid fast bacteria belonging to the genus mycobacterium, which include the causative agent for tuberculosis.
This file is licensed under the creative commons attributionshare alike 4. Paul ehrlich is the first to describe the acid fast properties of the bacterium. Links to pubmed are also available for selected references. While acid fast and gram staining are both differential stains, the acid fast stain is much more specific. To prepare the bacteria culture slides, a volume of 1 ml of bacterial. Currently in most laboratories, methods of staining using fluorescence microscopy fm and lightemitting diodes led microscopy for acid fast bacilli afb are. Choose from 500 different sets of acid fast staining microbiology flashcards on quizlet. In ziehl neelsen procedure10, smeared slides are primarily stained with. Carbolfuchsin acid fast stain is used to demonstrate mycobacterium tu ber cu lo sis in tissue sections and on smears. It is the differential staining techniques which was first developed by ziehl and later on modified by neelsen.